Chapter 2 — Thiol-Reactive Probes

Table of Contents 

Section 2.1 — Introduction to Thiol Modification and Detection 

• Common Applications for Thiol-Reactive Probes
• Labeling Biopolymers
• Derivatizing Low Molecular Weight Molecules
• Quantitating Thiols
• Reactivity of Thiol Groups
• Iodoacetamides
• Maleimides
• Other Thiol-Reactive Reagents
• Reagents for Quantitating Thiols
• Thiol and Sulfide Quantitation Kit
• Ellman's Reagent (DTNB) for Quantitating Thiols
• Fluorometric Reagents for Quantitating Thiols
• Data Table
• Product List

Section 2.2 — Thiol-Reactive Probes Excited with Visible Light 

• BODIPY Derivatives
• BODIPY Maleimides, Iodoacetamides and Methyl Bromides
• BODIPY FL L-Cystine
• Alexa Fluor Derivatives
• Fluorescein Derivatives, Including Thiol-Reactive Oregon Green Dyes
• Fluorescein Derivatives
• Oregon Green Derivatives
• Eosin Derivatives
• Rhodamine Derivatives, Including Thiol-Reactive Texas Red Dyes
• Tetramethylrhodamine Derivatives
• A Rhodamine-Based Crosslinking Reagent
• Rhodamine Red Derivative
• Texas Red Derivatives
• Pyridyloxazole Derivatives
• Benzoxadiazole Derivatives, Including NBD Probes
• NBD Chloride and NBD Fluoride
• SBD Probes
• IANBD Ester and IANBD Amide
• Lucifer Yellow Iodoacetamide
• Chromophoric Maleimides and Iodoacetamides
• Labeling Cell-Surface Thiols and Disulfides
• NANOGOLD Monomaleimide
• Data Table
• Product List

Section 2.3 — Thiol-Reactive Probes Excited with Ultraviolet Light 

• Coumarin Derivatives
• Alexa Fluor 350 C₅ Maleimide
• Other Coumarin Maleimides and Iodoacetamides
• Naphthalene Derivatives, Including Thiol-Specific Didansyl Cystine
• Acrylodan and Its Bromoacetyl Analog
• IAANS and MIANS
• IAEDANS
• Dansyl Aziridine
• N,N'-Didansyl-L-Cystine
• Dapoxyl Derivatives
• Pyrene Derivatives
• Pyrene Maleimide
• Pyrene Iodoacetyl Derivatives
• Bimanes for Thiol Derivatization
• Polar Reagents for Determining Thiol Accessibility
• 1,10-Phenanthroline Iodoacetamide for Preparing Metal-Binding Conjugates
• Data Table
• Product List