Chapter 1 — Fluorophores and Their Amine-Reactive Derivatives
- Common Applications for Amine-Reactive Probes
- Labeling Biopolymers
- Preparing the Optimal Bioconjugate
- Derivatizing Low Molecular Weight Molecules
- Reactivity of Amino Groups
- Isothiocyanates
- Succinimidyl Esters and Carboxylic Acids
- Sulfonyl Chlorides
- Other Amine-Reactive Reagents
- Kits for Labeling Proteins with a Fluorescent Dye or Biotin
- FluoReporter Protein Labeling Kits
- Easy-to-Use Protein Labeling Kits
- Monoclonal Antibody Labeling Kits
- FluoReporter Biotin-XX Protein Labeling Kit
- FluoReporter Mini-Biotin-XX Protein Labeling Kit
- DSB-X Biotin Protein Labeling Kit
- FluoReporter Biotin/DNP Protein Labeling Kit
- Zenon One Mouse IgG1 Labeling Kits
- Nucleic Acid Labeling Kits
- ARES DNA Labeling Kits
- Alexa Fluor Oligonucleotide Amine Labeling Kits
- ULYSIS Nucleic Acid Labeling Kits
- Product List
- Features of the Alexa Fluor Dyes
- Alexa Fluor 488 Dye
- Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 568 and Alexa Fluor 594 Dyes
- Alexa Fluor 633, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 700 and Alexa Fluor 750 Dyes
- Alexa Fluor 350 Dye
- Alexa Fluor 430 Dye
- Alexa Fluor Labeling Reagents and Kits
- Alexa Fluor Bioconjugates
- Alexa Fluor Tandem Conjugates of Phycobiliproteins
- Signal Amplification with Alexa Fluor Dyes
- Tyramide Signal Amplification
- Antibody-Based Signal Amplification Kits
- Alexa Fluor Conjugates of Anti-Fluorescein/Oregon Green
- Antibody to the Alexa Fluor 488 Dye
- Data Table
- Product List
- Overview of Our BODIPY Fluorophores
- BODIPY FL Dye: A Substitute for Fluorescein
- Longer-Wavelength BODIPY Dyes
- Amine-Reactive BODIPY Dyes
- BODIPY Dye Succinimidyl Esters
- Water-Soluble BODIPY FL Succinimidyl Esters and STP Esters
- BODIPY Carboxylic Acids
- BODIPY Dye Conjugates
- Peptides and Proteins
- BODIPY Dye Conjugates of Nucleotides and Oligonucleotides
- BODIPY Dye Conjugates of Lipids and Receptor Ligands
- BODIPY Dye Conjugates as Enzyme Substrates and for High-Throughput Screening Applications
- EnzChek Kits and DQ Reagents as Fluorogenic Enzyme Substrates
- EnzChek Polarization Assay Kit for Proteases
- Lipophilic BODIPY Substrates for Phospholipases and Other Enzymes
- BODIPY Dye–Based Substrates for Chloramphenicol Acetyltransferase
- BODIPY Dye–Labeled Nucleotides as Enzyme Substrates and for High-Throughput Screening Applications
- Conjugates of BODIPY Dyes for Fluorescence Polarization–Based Assays
- Additional Methods of Analysis Using BODIPY Dye Conjugates
- Data Table
- Product List
- Spectral Properties of Fluorescein
- Limitations of Fluoresceins
- Reactive Derivatives of Fluorescein
- Single-Isomer Fluorescein Isothiocyanate (FITC) Preparations
- Mixed-Isomer and Single-Isomer Preparations of Carboxyfluorescein (FAM) Succinimidyl Ester
- Succinimidyl Esters of Fluorescein with Spacer Groups
- Fluorescein Dichlorotriazine (DTAF)
- Caged Fluorescein
- Oregon Green 488 and Oregon Green 514 Dyes
- Spectral Properties of the Oregon Green Dyes
- Advantages of the Oregon Green Dyes
- Reactive Oregon Green Dyes
- Oregon Green Protein and Nucleic Acid Labeling Kits
- Oregon Green 488 Tyramide Signal Amplification Kits
- Conjugates of Oregon Green Dyes
- Fluorescein Derivatives for Genetic Analysis
- Eosins and Erythrosins: Phosphorescent Probes and Photosensitizers
- Eosin and Erythrosin
- An Eosin Analog
- Rhodamine Green Dyes
- Reactive Rhodamine Green Dyes
- Rhodamine Green Conjugates
- Data Table
- Product List
- Tetramethylrhodamine
- Mixed-Isomer and Single-Isomer TRITC Preparations
- Succinimidyl Esters of Carboxytetramethylrhodamine (TAMRA)
- Lissamine Rhodamine B and Rhodamine Red-X Dyes
- Lissamine Rhodamine B Sulfonyl Chloride
- Rhodamine Red-X Succinimidyl Ester
- X-Rhodamine
- Texas Red and Texas Red-X Dyes
- Texas Red Sulfonyl Chloride
- Texas Red-X Succinimidyl Ester
- Texas Red-X STP Ester
- Texas Red-X Conjugates and Texas Red-X Labeling Kits
- Naphthofluorescein
- Carboxyrhodamine 6G
- QSY Dyes: The Best Fluorescence Quenchers
- Nonfluorescent Malachite Green
- NANOGOLD Sulfosuccinimidyl Ester
- Data Table
- Product List
- Cascade Blue Dye
- Coumarin Derivatives
- Alexa Fluor 350 and AMCA-X Dyes
- Alexa Fluor 430 Dye
- Alexa Fluor and Zenon One Labeling Kits
- Marina Blue and Pacific Blue Dyes
- Pacific Blue Tyramide Signal Amplification Kits
- Zenon One Labeling Kits with the Marina Blue and Pacific Blue Dyes
- Alexa Fluor 350 and Pacific Blue Nucleic Acid Labeling Kits
- Other Hydroxycoumarin and Alkoxycoumarin Derivatives
- Naphthalenes, Including Dansyl Chloride
- Pyrenes
- Pyridyloxazole Derivatives
- Cascade Yellow Dye
- Dapoxyl Dye
- UV Light–Excitable Microspheres
- Data Table
- Product List
- Fluorescamine
- Dialdehydes: OPA and NDA
- Analyte Detection with OPA and NDA
- Sensitivity of NDA
- Applications for OPA and NDA
- ATTO-TAG Reagents
- Sensitivity of ATTO-TAG CBQCA and ATTO-TAG FQ
- ATTO-TAG Reagents and Kits
- 7-Nitrobenz-2-Oxa-1,3-Diazole (NBD) Derivatives
- Dansyl Chloride and Other Sulfonyl Chlorides
- Dansyl Chloride
- Dapoxyl Sulfonyl Chloride
- Pyrene Sulfonyl Chloride
- Chromophoric Sulfonyl Chloride
- FITC and Benzofuran Isothiocyanates
- Succinimidyl Esters and Carboxylic Acids
- The Smallest Reactive Fluorophore
- Chromophoric Succinimidyl Esters: Fluorescence Quenchers
- Biotinylation, Desthiobiotinylation, Crosslinking and Thiolation Reagents
- Data Table
- Product List
