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NICE® Expression System for Lactococcus lactis

  
 

The effective & easy-to-operate NIsin Controlled gene Expression system

  
     

Controlled gene expression in L. lactis – an emerging alternative for recombinant protein production

      
 
NICE® System
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B. megaterium
B. subtilis
 

 

Next to the wealth of traditional food applications, L. lactis is increasingly used for modern biotechnological applications such as the production of recombinant proteins for food, feed, pharma and biocatalysis applications. Next to the easy genetic accessibility of L. lactis the Nisin Controlled gene Expression (NICE®) system developed at NIZO has played a key role in this development.

Features

  • The system is fully food grade
  • No endotoxins are produced
  • No inclusion bodies
  • No spores
  • No extracellular proteinases
  • Tightly controlled gene expression allows production of toxic proteins
  • Simple fermentation, scale-up and down stream processing

Areas of application

  • Production of homologous and heterologous proteins for food, feed, pharma and biocatalysis applications
  • Production of prokaryotic and eukaryotic membrane proteins
  • Production of exo-polysaccharides
  • Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
  • Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as e.g. dehydrogenases and in-situ co-factor regenearation
  • High throughput screening for enzyme evolution or enzyme comparison.

     
     

Order Information

Please click on the respective order # for further details.
ORDER INFORMATION, SHIPPING & STORAGE:
order# description amount
Vector Map
Sequence
VS-ELV00100-01 NICE® pNZ8008 Reference plasmid with gusA gene 10 µg map txt
VS-ELV00200-01 NICE® pNZ8148 Lactococcus lactis expression vector, NcoI site 10 µg map txt
VS-ELV00250-01 NICE® pNZ8150 Lactococcus lactis expression vector, ScaI site 10 µg map txt
VS-ELV00300-01 NICE® pNZ8149 Lactococcus lactis expression vector, food grade, NcoI site 10 µg map txt
shipped at RT, store at -20 °C      
       
VS-ELV00500-01 NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000 1 ml map txt
VS-ELS09000-01 NICE® Lactococcus lactis expression Strain NZ9000 1 ml    
VS-ELS03900-01 NICE® Lactococcus lactis expression Strain NZ3900, food grade 1 ml    
shipped on dry ice, store at -80 °C      
       
VS-ELK01000-01 NICE® Nisin kit (50 mg Nisin, 1 ml 5% acetic acid) 1 kit    
shipped at RT, store at 4 °C
     
     
           
     

Downloads

NICE® Expression System for Lactococcus lactis Handbook (PDF, 666 kb, update 06.2010)

For further Vector System products please download the Vector Systems brochure (PDF, 3,9 MB)

  
             
     

Literature

  • de Ruyter, P. G., O. P. Kuipers, et al. (1996). "Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis." Journal of Bacteriology 178(12): 3434-3439. de Vos, W. M. (1987). "Gene cloning and expression in lactic streptococci."
    FEMS Microbiology Letters 46: 281-295. Pubmed

  • Frelet-Barrand, A., Boutigny, S., Kunji, E. R. S., und Rolland, N. (2010). "Membrane protein expression in Lactococcus lactis."
    Methods Mol. Biol 601, 67-85.

  • Gasson, M. J. (1983). "Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing."
    Journal of Bacteriology 154(1): 1-9. Pubmed

  • Kleerebezem, M., M. M. Beerthuyzen, et al. (1997). "Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp."
    Applied and Environmental Microbiology 63(11): 4581-4584. Pubmed

  • Kuipers, O. P., M. M. Beerthuyzen, et al. (1993). "Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity."
    European Journal of Biochemistry 216(1): 281-291. Pubmed

  • Mierau, I. and M. Kleerebezem (2005). "10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis."
    Applied Microbiology and Biotechnology 9: 1-13. Pubmed

  • Mierau, I., P. Leij, et al. (2005). "Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: the case of lysostaphin."
    Microbial Cell Factories 4: 15. Pubmed

  • Simon, D. and A. Chopin (1988). "Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis."
    Biochimie 70(4): 559-566. Pubmed

      
           
     


nizo
The patented NICE® system was developed by NIZO food research BV. NICE® is a trademark of NIZO food research BV. Patent EP0712935, EP0355036, EP0228726.

      
 
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