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Click-Chemistry |
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The new innovative system for easy labeling of DNA and RNA! |
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Features
- Fast and purposeful chemical reaction with high yields
- Convenient labeling of RNA or DNA via solid-phase synthesis (Phosphoramidites) or via PCR with high efficiency
- Up to three different modifications onto one position of the same DNA strand
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Description
Click-chemistry was first introduced by K. Berry Sharpless in 2001. It describes a chemical reaction which builds substances by joining small units together. Click-reactions are fast and purposeful with high yields.
The most popular click-reaction is azide alkyne Huisgen cycloaddition with Copper (Cu) as catalyst at room temperature.
Now you can use this highly efficient technology for labeling of DNA or RNA via solid-phase synthesis or PCR. Furthermore, it is possible to introduce up to three different modifications onto one position of the same DNA strand. Of course, we offer different kinds of non-fluorescent and fluorescent labels.
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Fig. 1 Schematic overview of solid phase synthesis
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| Fig. 2 DNA can be modified postsynthetically using click chemistry. The attachment of up to three different labels is possible on any position of the DNA strands, multiple functionalizations with the same labeling moiety is possible as well. |
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For labeling via solid-phase synthesis (Fig. 1) MoBiTec offers Phosphoramidites which enable the synthesis of oligonucleotides with reactive triple bonds for the attachment of different labels.
Another possibility is the generation of DNA strands with reactive triple bonds by PCR through integration of our C8-Alkyne triphosphates.
For labeling you can use either non- fluorescent labels like Biotin- or Dabcylazides or fluorescent azides/NHS. MoBiTec offers a great range of different fluorescent dyes for click-chemistry. In addition to Fluorescein-Azide we supply various MFP-Eterneon-Azides/-NHS. Our MFP-Eterneon-Dyes have a low molecular weight and good solubility. Most notably, with their great Stokes shift they are convenient for cell cytometry.
Ancillary material like CuBr or Click-Solution is available as well. |
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Order Information, Shipping & Storage
Phosphoramidites
| Order Information |
| Order # |
Description |
Amount |
| MFPCCA-07 |
C8-Alkyne-dC-CEP |
250 mg |
| MFPCCA-03 |
C8-Alkyne-dU-CEP |
250 mg |
| MFPCCA-08 |
C8-TIPS-dC-CEP |
250 mg |
Triphosphates
Azides
Fluorescence Dyes
Miscellaneous
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Literature
- Burley, G. A., Gierlich, J., Mofid, M. R., Nir, H., Tal, S., Eichen, Y., und Carell, T. (2006). Directed DNA metallization. J. Am. Chem. Soc 128, 1398–1399.
- Gramlich, P. M. E., Warncke, S., Gierlich, J., und Carell, T. (2008). Click-Click-Click: Single to Triple Modification of DNA13. Angewandte Chemie International Edition 47, 3442-3444.
- Gramlich, P. M. E., Wirges, C., Manetto, A., und Carell, T. (2008). Postsynthetic DNA Modification through the Copper-Catalyzed Azide-Alkyne Cycloaddition Reaction. Angewandte Chemie International Edition 47, 8350-8358.
- Kolb, H. C., Finn, M. G., und Sharpless, K. B. (2001). Click Chemistry: Diverse Chemical Function from a Few Good Reactions. Angewandte Chemie International Edition 40, 2004-2021.
- Seela, F., Sirivolu, V. R., und Chittepu, P. (2008). Modification of DNA with Octadiynyl Side Chains: Synthesis, Base Pairing, and Formation of Fluorescent Coumarin Dye Conjugates of Four Nucleobases by the Alkyne−Azide “Click” Reaction. Bioconjugate Chemistry 19, 211-224.
- Seela, F., Xiong, H., Leonard, P., und Budow, S. (2009). 8-Aza-7-deazaguanine Nucleosides and Oligonucleotides with Octadiynyl Side Chains: Synthesis, Functionalization by the Azide-Alkyne 'Click' Reaction and Nucleobase specific Fluorescence Quenching of Coumarin dye conjugates. Org. Biomol. Chem 7, 1374-1387.
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Downloads
Please download Click Chemistry Manual (pdf, 138 KB) |
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| ©2010 MoBiTec GmbH • All rights reserved • www.mobitec.com |
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