Products | Proteomics

 

Optimal Protein staining

 
   
 
button Fluorescent protein staining
button SYPRO® Ruby
button SYPRO® Orange, SYPRO® Red and SYPRO® Tangerine
button SYPRO® Ruby and SYPRO® Rose Plus Blot Stains
button DyeChrome™ Two-Color Fluorescent Staining of Western Blots
button Pro-Q™ Western Blot Stain Kits
button Pro-Q™ Emerald Stain Kits - Efficient Glycoprotein Detection
button Pro-Q™ Sapphire His-Tag
button Conventional protein staining
button Deep Blue - Colloidal Coomassie
button Deep Silver Stain
button Snow White Reversible Zinc Stain
button Enlight Fluorographic Enhancer
button NOWA Chemiluminescent Western
 


   
 

Fluorescent Protein Gel and Blot Stains

SYPRO® Ruby - highly efficient one-step protein staining in 1D, 2D and IEF gels

 
 
Features:
  • Sensitivity rivaling the best silver stains
  • No stop solutions or destaining required
  • Compatible with mass spectrometry and microsequencing
  • Broad linear range for accurate comparisons
  • Stains even minimally expressed proteins in 2D gels
 
SYPRO Ruby   Silverstain vs. SYPRO RubySYPRO Ruby
Amounts of carbonic anhydrase ranging from 1 ng to 1000 ng were separated by SDS-PAGE and stained with SYPRO® Ruby Protein Gel Stain.   Fig1: SYPRO® Ruby Protein Gel Stain compared to silver stain. Proteins from a fibroblast cell lysate were run on a 2D gel and stained with SYPRO® Ruby Protein Gel Stain (top) or silver stain (bottom). Fig2: Protein molecular weight markers stained with SYPRO® Ruby Protein Gel Stain.
 
 
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SYPRO® Orange, SYPRO® Red and SYPRO® Tangerine - easy-to-use stains for detection of proteins in SDS-PAGE

 
 
Features:
  • High-sensitivity detection (4 - 8 ng/band)
  • One-step procedure takes less than an hour -requires no stop solutions or destaining steps
  • Broad linear quantitation range
  • Can be visualized with UV transilluminators, laser scanners or our Dark Reader™
  • SYPRO® Tangerine stain is ideal for zymography, electroelution and blotting applications

     
  SYPRO staining

Staining with SYPRO® gel stains is simple - just fix, stain and wash
     
 
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SYPRO® Ruby and SYPRO® Rose Plus Blot Stains - detection of total proteins on blotting membranes

 
 
Features:
  • High sensitivity detection (down to ~2 - 8 ng protein/band or ~0.25 - 1 ng protein/mm2 )
  • Ready-to-use, simple staining solution
  • Can be visualized with UV transilluminators, laser scanners or our Dark Reader™
  • Compatible with subsequent immunostaining or lectin staining, Edman sequencing or mass spectrometry
  • SYPRO® Rose Plus stains proteins in a reversible manner, has a particularly long fluorescence lifetime and is very useful for temporary protein detection on solid supports, such as contact lenses or protein chips
     
 
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DyeChrome™ - Two-Color Fluorescent Staining of Western Blots

 
 
Features:
  • Two-color fluorescent staining with total protein and immunostained protein visible in contrasting colors on the same blot - eliminates uncertainties inherent in comparing duplicate blots
  • Low nanogram sensitivity limits for both staining techniques
  • Stains can be seen simultaneously under UV light and documented separately using appropriate excitation light sources and emission filters

 
Protein detection with DyeChromex Western Blot Stain Kit.
Protein detection with DyeChrome™ Western Blot Stain Kit. Samples containing decreasing amounts of protein molecular weight standards were run on two identical SDS-polyacrylamide gels and blotted onto a PVDF membrane. The blot was stained with BODIPY® TR-X succinimidyl ester (red) to detect total protein. Then it was incubated with anti-alpha -tubulin antibody, followed by the alkaline phosphatase conjugate of goat anti-mouse IgG, which is included in the kit. Finally, the blot was incubated with ELF® 39 phosphate (green). The fluorescent signals were visualized using UV epi-illumination, documented separately using appropriate filters (A and B), and the resulting images overlaid (C).

 
 
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Pro-Q™ Western Blot Stain Kits - detection of total protein and immuno-stained proteins on the same blot

 
 
Features:
  • High-sensitivity detection of alkaline phosphatase conjugates with DDAO phosphate
  • Rivaling the best chemiluminescent staining
  • Darkroom conditions and X-ray film not required
  • Staining can be imaged several times and is stable on dried blots
  • Compatible with SYPRO® Ruby Protein Blot Stain
  • Can be visualized with UV transilluminator, laser scanners or our Dark Reader™

 
Protein detection using Pro-Qx Western   Schematic diagram of Western blot immunodetection with DDAO phosphate.
Protein detection using Pro-Q™ Western Blot Stain Kits.   Schematic diagram of Western blot immunodetection with DDAO phosphate.
 
 
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Pro-Q™ Emerald Stain Kits - efficient glycoprotein detection in gels or on blots

 
 
Features:
  • Three-step staining is completed in less than 3 hours
  • More sensitive than other glycoprotein stainings
  • Compatible with SYPRO® Ruby Protein Stain for dual detection
 
Mobility-shift assay with Pro-Qx Emerald 300 and SYPRO® Ruby   Mobility-shift assay using deglycosylated enzymes. Gels were stained with either Pro-Q™ Emerald 300 (bottom) or SYPRO® Ruby Protein Gel Stain
(top). The CandyCane™ glycoprotein molecular weight standards were loaded into lanes 1 and 8.
 
 
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Pro-Q™ and Pro-Q™ Sapphire His-Tag Blot and His-Tag Gel Stains -
for easy and sensitive detection of His-tags

 
 
Features:
  • One-step gel staining procedure requires very little hands-on time arrowcompleted in just a few hours
  • Detect as little as 25 ng of His-tagged proteins directly in the gel and as little as 16 ng of His- tagged protein immobilized on PVDF membranes
  • Blot staining is completed in less than 90 minutes
  • Reversible binding allows the blot to be analyzed with another antibody
     
 
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Order Information

Click on the respective order # to download a detailed data sheet.
ORDER INFORMATION:
order# description amount
S11791 SYPRO® Ruby protein blot stain *10-40 blots* 200 mL
S12000 SYPRO® Ruby protein gel stain 1 L
S12001 SYPRO® Ruby protein gel stain 200 mL
S21900 SYPRO® Ruby protein gel stain *bulk packaging* 5 L
S6650 SYPRO® Orange protein gel stain *5000X concentrate in DMSO* 500 µL
S6651 SYPRO® Orange protein gel stain *5000X concentrate in DMSO* *special packaging* 10 x 50 µL
S6653 SYPRO® Red protein gel stain *5000X concentrate in DMSO* 500 µL
S6654 SYPRO® Red protein gel stain *5000X concentrate in DMSO* *special packaging* 10 x 50 µL
S12010 SYPRO® Tangerine protein gel stain *5000X concentrate in DMSO* 500 µL
S12011 SYPRO® Rose Plus Protein Blot Stain Kit *10-40 blots* 1 kit
S12012 SYPRO® Protein Gel Stain Starter Kit 1 kit
D21883 DyeChrome™ Western Blot Stain Kit #3 *with streptavidin, DDAO phosphate and BODIPY® FL-X, SE* *20 minigel blots* 1 kit
D21887 DyeChrome™ Double Western Blot Stain Kit *for mouse IgG, rabbit IgG and total protein detection* *20 minigel blots* 1 kit
P33203 Pro-Q® Diamond LC Phosphopeptide Detection Kit 1 kit
P33300 Pro-Q® Diamond phosphoprotein gel stain 1 L
MPP33300 Pro-Q® Diamond Phosphoprotein Gel Staining Kit *includes 1 L stain and 40 µL standard* 1 kit
P33301 Pro-Q® Diamond phosphoprotein gel stain 200 mL
MPP33301 Pro-Q® Diamond Phosphoprotein Gel Staining Kit *includes 200 mL stain and 40 µL standard* 1 kit
P33302 Pro-Q® Diamond phosphoprotein gel stain *bulk packaging* 5 L
MPP33302 Pro-Q® Diamond Phosphoprotein Gel Staining Kit *includes 5 L stain and 400 µL standard* 1 kit
P33310 Pro-Q® Diamond phosphoprotein gel destaining solution 1 L
P33311 Pro-Q® Diamond phosphoprotein gel destaining solution *bulk packaging* 5 L
P33356 Pro-Q® Diamond Phosphoprotein Blot Stain Kit *20 minigel blots* 1 kit
P33358 Pro-Q® Diamond Phosphoprotein Enrichment Kit 1 kit
P33359 Pro-Q® Diamond Phosphoprotein Enrichment and Detection Kit 1 kit
P33706 Pro-Q® Diamond Phosphoprotein/Phosphopeptide Microarray Stain Kit 1 kit
P20495 Pro-Q® Emerald 300 Lipopolysaccharide Gel Stain Kit *10 minigels* 1 kit
P21855 Pro-Q® Emerald 300 Glycoprotein Gel Stain Kit *with SYPRO® Ruby protein gel stain* *10 minigels* 1 kit
P21857 Pro-Q® Emerald 300 Glycoprotein Gel and Blot Stain Kit *10 minigels or minigel blots* 1 kit
P21875 Pro-Q® Emerald 488 Glycoprotein Gel and Blot Stain Kit *10 minigels or minigel blots* 1 kit
P21876 Pro-Q® Sapphire 365 oligohistidine gel stain *20 minigels* 500 mL
P21877 Pro-Q® Sapphire 488 oligohistidine gel stain *20 minigels* 500 mL

Distributed for Molecular Probes

 
 


 
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Conventional protein staining

Deep Blue - Colloidal Coomassie

 
 
Product description:

Deep Blue is the easiest way to Coomassie stain your protein gels. It´s sensitive, it doesn´t smell, it´s fast and it´s destain free! The equilibrium of the blue dye in Deep Blue is primarily driven towards its solid colloid state, leaving the solutuion almost colorless. Protein bands inside a gel accumulate these trace amounts of dye quickly and develop a dark blue stain while the rest of the gel remains clear. Watch your bands develop in front of your eyes, directly in the staining tray. After one hour the staining is complete and the gel can be dried directly. It is also possible to further increase the sensitivity of Deep Blue by an additional water wash step to detect even the weakest bands. Deep Blue offers the best results for the best price!
The reusable Deep Blue is stable at room temperature for at least one year.

 
stained with Deep Blue   stained with conventional Coomassie Blue + destain
Two identical SDS polyacrylamide gels stained with Deep Blue (A) and conventional Coomassie Blue + destain (B).
 
 
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Deep Silver Stain - High-sensitive gel staining with one single solution!

 
 

Features:

  • Highly improved contrast and sensitivity (< 200 pg)
  • Very fast and easy 30-minutes procedure!
  • Suitable for 2D and DNA gels
 
  Example of a Deep Silver
Example of a Deep Silver stained SDS-PAGE gel.
 
 
Product description:

Deep Silver combines the sensitivity of conventional silver staining with the speed and ease you 've always been waiting for (see your bands 30 minutes after fixing). Whereas former protocols require multiple incubation, washing and development steps, Deep Silver does it all in one single solution! Moreover, contrast and sensitivity are markedly improved with this kit (down to 200 pg! ). While you got brownish bands on your gels until now, Deep Silver generates a deep black staining color. The silver stain develops over a period of 10 - 20 minutes, to give you enough time to stop the reaction at the point of staining intensity you require. The reaction is stopped in EDTA or standard fixing solution and gels can be dried and stored as usual. Deep Silver is suitable for the staining of 2D and DNA gels as well. One kit is sufficient for 40 SDS mini gels.

 
 
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Snow White Reversible Zinc Stain - Rapid negative stain for SDS gels

 
 
Features:
  • Crystal clear protein bands
  • Fast and simple two-step staining procedure
  • Stained gel can be used for many subsequent procedures such as Western blotting, amino acid analysis, electroelution and MALDI-TOF
 
Example of a Snow White stained SDS-PAGE gel placed against the dark background of a UV-transilluminator.
Example of a Snow White stained SDS-PAGE gel placed against the dark background of a UV-transilluminator.
 
 
Product description:

Snow White is a reversible, rapid negative stain for SDS-PAGE gels. A simple two-step staining procedure allows visualization of bands in just 10 minutes with a sensitivity close to that of silver stain. Snow White produces an opaque white background with crystal clear protein bands that can easily be viewed and documented when placed against a dark background (e.g. a UV screen). The destaining solution of the kit allows complete destaining of the gel in just 15 minutes. Gels can then be applied to subsequent procedures such as Western blotting, electroelution, amino acid analysis, N-terminal sequencing, MALDI-TOF...you name it!

 
 
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Enlight Fluorographic Enhancer - fluorographic enhancement of low-energy isotopes in polyacrylamide and agarose gels

 
 
Product description:

Enlight is a non-toxic aqueous solution that allows the fluorographic enhancement of low-energy isotopes in polyacrylamide and agarose gels. After electrophoresis, the gel is soaked in the Enlight solution for one hour. Then, the gel is dried as usual and exposed to an X-ray film at -70°C. Low-energy electrons that cannot leave the gel matrix are likely to interact with fluorographic molecules which are now incorporated in the gel and stimulate a light-emitting process. These photons can easily leave the gel to expose the film. The increased detection efficiency for 3H is at least 10,000 fold, for 14C, 35S and 33P at least 100-fold! Enlight is stable at room temperature for at least 12 months.

  Comparison of Enlight-treated and -un-treated gels with 14C-labeled protein marker and 3H- and 35S-labeled cell lysates after overnight exposure at -70°C.
Comparison of Enlight-treated and -un-treated gels with 14C-labeled protein marker and 3H- and 35S-labeled cell lysates after overnight exposure at -70°C.
 
 
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NOWA Chemiluminescent Western - Highly sensitive detection system for
HRP-conjugated antibodies

 
 
Product description:

NOWA (New Optical Western Analysis) is a highly sensitive detection system for HRP-conjugated antibodies in the picogram range. It enables a sensitivity that is significantly higher than that of other leading chemiluminescence kits.

 
Comparison of light emission of NOWA and popular supplier A´s kit.
Comparison of light emission of NOWA and popular supplier A´s kit.

 
  The kit is based on the emission of light during a reaction which is started by free oxygen produced by the enzymatic activity of HRP. Using a newly developed enhancer cascade, the light emission is amplified by a factor of at least one thousand! The NOWA kit contains two solutions that are combined and used for incubating the nitrocellulose or PVDF filter. After one minute of incubation time the filter is ready to be exposed to an X-ray film. Typical exposure times range between 10 seconds and 5 minutes. The light-emitting reaction continues for about 1 hour to allow as many exposures as you may need. The shelf life of the NOWA kit is at least 12 months when stored at 4°C. The kit contains 2 x 250 ml and is sufficient for 10,000 cm2 (1 m2 ) of NC or PVDF membrane.
 
 
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Order Information

Click on the respective order # to download a detailed data sheet.
ORDER INFORMATION, SHIPPING & STORAGE:
order# description amount
DB001 Deep Blue (50 mini gels) 0.5 l
DB002 Deep Blue (100 mini gels) 1 l
SS001 Deep Silver Kit (40 mini gels) 1
shipped at RT; store at 4°C (Deep Blue can be stored at RT)
SW001 Snow White Kit (25 mini gels) 1
ENL01 Enlight 0.5 l
ENL02 Enlight 1 l
NOWA01 NOWA Kit (2 x 250 ml) 1
shipped at RT; store at RT (NOWA can be stored at 4°C)


 
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