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Optimal Protein staining |
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Fluorescent Protein Gel and Blot Stains
SYPRO® Ruby - highly efficient one-step protein staining in 1D, 2D and IEF gels
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Features:
- Sensitivity rivaling the best silver stains
- No stop solutions or destaining required
- Compatible with mass spectrometry and microsequencing
- Broad linear range for accurate comparisons
- Stains even minimally expressed proteins in 2D gels
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| Amounts of carbonic anhydrase ranging from
1 ng to 1000 ng were separated by SDS-PAGE
and stained with SYPRO® Ruby Protein Gel Stain. |
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Fig1: SYPRO® Ruby Protein Gel Stain compared to silver stain. Proteins from a fibroblast cell lysate were run on a 2D gel and stained with SYPRO® Ruby Protein Gel Stain (top) or silver stain (bottom). Fig2: Protein molecular weight markers stained with SYPRO® Ruby Protein Gel Stain. |
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Product list with data sheets
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SYPRO® Orange, SYPRO® Red and SYPRO® Tangerine
- easy-to-use stains for detection of proteins in SDS-PAGE
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Features:
- High-sensitivity detection (4 - 8 ng/band)
- One-step procedure takes less than an hour -requires no stop solutions or destaining steps
- Broad linear quantitation range
- Can be visualized with UV transilluminators, laser scanners or our Dark Reader™
- SYPRO® Tangerine stain is ideal for zymography, electroelution and blotting applications
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Staining with SYPRO® gel stains is simple - just fix, stain and wash |
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Product list with data sheets
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SYPRO® Ruby and SYPRO® Rose Plus Blot Stains - detection of total proteins on blotting membranes
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Features:
- High sensitivity detection (down to ~2 - 8 ng protein/band or ~0.25 - 1 ng protein/mm2 )
- Ready-to-use, simple staining solution
- Can be visualized with UV transilluminators, laser scanners or our Dark Reader™
- Compatible with subsequent immunostaining or lectin staining, Edman sequencing or mass spectrometry
- SYPRO® Rose Plus stains proteins in a reversible manner, has a particularly long fluorescence lifetime and is very useful for temporary protein detection on solid supports, such as contact lenses or protein chips
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Product list with data sheets
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DyeChrome™ - Two-Color Fluorescent Staining of Western Blots
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Features:
- Two-color fluorescent staining with total protein and immunostained protein visible in contrasting colors on the same blot - eliminates uncertainties inherent in comparing duplicate blots
- Low nanogram sensitivity limits for both staining techniques
- Stains can be seen simultaneously under UV light and documented separately using appropriate excitation light sources and emission filters
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| Protein detection with DyeChrome™ Western Blot Stain Kit. Samples containing decreasing amounts of protein molecular weight standards were run on two identical SDS-polyacrylamide gels and blotted onto a PVDF membrane. The blot was stained with BODIPY® TR-X succinimidyl ester (red) to detect total protein. Then it was incubated with anti-alpha -tubulin antibody, followed by the alkaline phosphatase conjugate of goat anti-mouse IgG, which is included in the kit. Finally, the blot was incubated with ELF® 39 phosphate (green). The fluorescent signals were visualized using UV epi-illumination, documented separately using appropriate filters (A and B), and the resulting images overlaid (C). |
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Product list with data sheets
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Pro-Q™ Western Blot Stain Kits - detection of total protein and immuno-stained proteins on the same blot
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Features:
- High-sensitivity detection of alkaline phosphatase conjugates with DDAO phosphate
- Rivaling the best chemiluminescent staining
- Darkroom conditions and X-ray film not required
- Staining can be imaged several times and is stable on dried blots
- Compatible with SYPRO® Ruby Protein Blot Stain
- Can be visualized with UV transilluminator, laser scanners or our Dark Reader™
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| Protein detection using Pro-Q™ Western
Blot Stain Kits. |
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Schematic diagram of Western blot immunodetection with DDAO phosphate. |
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Product list with data sheets
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Pro-Q™ Emerald Stain Kits - efficient glycoprotein detection in gels or on blots
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Features:
- Three-step staining is completed in less than 3 hours
- More sensitive than other glycoprotein stainings
- Compatible with SYPRO® Ruby Protein Stain for dual detection
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Mobility-shift assay using deglycosylated enzymes. Gels were stained with either Pro-Q™ Emerald 300 (bottom) or SYPRO® Ruby Protein Gel Stain
(top). The CandyCane™ glycoprotein molecular weight standards were loaded into lanes 1 and 8. |
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Product list with data sheets
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Pro-Q™ and Pro-Q™ Sapphire His-Tag Blot and His-Tag Gel Stains -
for easy and sensitive detection of His-tags
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Features:
- One-step gel staining procedure requires very little hands-on time
completed in just a few hours
- Detect as little as 25 ng of His-tagged proteins directly in the gel and as little as 16 ng of His- tagged protein immobilized on PVDF membranes
- Blot staining is completed in less than 90 minutes
- Reversible binding allows the blot to be analyzed with another antibody
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Product list with data sheets
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Order Information
| Click on the respective order # to download a detailed data sheet. |
| ORDER INFORMATION: |
| order# |
description |
amount |
S11791 |
SYPRO® Ruby protein blot stain *10-40 blots* |
200 mL |
| S12000 |
SYPRO® Ruby protein gel stain |
1 L |
| S12001 |
SYPRO® Ruby protein gel stain |
200 mL |
S21900 |
SYPRO® Ruby protein gel stain *bulk packaging* |
5 L |
| S6650 |
SYPRO® Orange protein gel stain *5000X concentrate in DMSO* |
500 µL |
| S6651 |
SYPRO® Orange protein gel stain *5000X concentrate in DMSO* *special packaging* |
10 x 50 µL |
| S6653 |
SYPRO® Red protein gel stain *5000X concentrate in DMSO* |
500 µL |
| S6654 |
SYPRO® Red protein gel stain *5000X concentrate in DMSO* *special packaging* |
10 x 50 µL |
| S12010 |
SYPRO® Tangerine protein gel stain *5000X concentrate in DMSO* |
500 µL |
| S12011 |
SYPRO® Rose Plus Protein Blot Stain Kit *10-40 blots* |
1 kit |
| S12012 |
SYPRO® Protein Gel Stain Starter Kit |
1 kit |
| D21883 |
DyeChrome™ Western Blot Stain Kit #3 *with streptavidin, DDAO phosphate and BODIPY® FL-X, SE* *20 minigel blots* |
1 kit |
| D21887 |
DyeChrome™ Double Western Blot Stain Kit *for mouse IgG, rabbit IgG and total protein detection* *20 minigel blots* |
1 kit |
P33203 |
Pro-Q® Diamond LC Phosphopeptide Detection Kit |
1 kit |
| P33300 |
Pro-Q® Diamond phosphoprotein gel stain |
1 L |
| MPP33300 |
Pro-Q® Diamond Phosphoprotein Gel Staining Kit *includes 1 L stain and 40 µL standard* |
1 kit |
| P33301 |
Pro-Q® Diamond phosphoprotein gel stain |
200 mL |
| MPP33301 |
Pro-Q® Diamond Phosphoprotein Gel Staining Kit *includes 200 mL stain and 40 µL standard* |
1 kit |
| P33302 |
Pro-Q® Diamond phosphoprotein gel stain *bulk packaging* |
5 L |
| MPP33302 |
Pro-Q® Diamond Phosphoprotein Gel Staining Kit *includes 5 L stain and 400 µL standard* |
1 kit |
| P33310 |
Pro-Q® Diamond phosphoprotein gel destaining solution |
1 L |
| P33311 |
Pro-Q® Diamond phosphoprotein gel destaining solution *bulk packaging* |
5 L |
P33356 |
Pro-Q® Diamond Phosphoprotein Blot Stain Kit *20 minigel blots* |
1 kit |
| P33358 |
Pro-Q® Diamond Phosphoprotein Enrichment Kit |
1 kit |
| P33359 |
Pro-Q® Diamond Phosphoprotein Enrichment and Detection Kit |
1 kit |
| P33706 |
Pro-Q® Diamond Phosphoprotein/Phosphopeptide Microarray Stain Kit |
1 kit |
P20495 |
Pro-Q® Emerald 300 Lipopolysaccharide Gel Stain Kit *10 minigels* |
1 kit |
| P21855 |
Pro-Q® Emerald 300 Glycoprotein Gel Stain Kit *with SYPRO® Ruby protein gel stain* *10 minigels* |
1 kit |
| P21857 |
Pro-Q® Emerald 300 Glycoprotein Gel and Blot Stain Kit *10 minigels or minigel blots* |
1 kit |
| P21875 |
Pro-Q® Emerald 488 Glycoprotein Gel and Blot Stain Kit *10 minigels or minigel blots* |
1 kit |
| P21876 |
Pro-Q® Sapphire 365 oligohistidine gel stain *20 minigels* |
500 mL |
| P21877 |
Pro-Q® Sapphire 488 oligohistidine gel stain *20 minigels* |
500 mL |
Distributed for
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Conventional protein staining
Deep Blue - Colloidal Coomassie
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Product description:
Deep Blue is the easiest way to Coomassie stain your protein gels. It´s sensitive, it doesn´t smell, it´s fast and it´s destain free! The equilibrium of the blue dye in Deep Blue is primarily driven towards its solid colloid state, leaving the solutuion almost colorless. Protein bands inside a gel accumulate these trace amounts of dye quickly and develop a dark blue stain while the rest of the gel remains clear. Watch your bands develop in front of your eyes, directly in the staining tray. After one hour the staining is complete and the gel can be dried directly. It is also possible to further increase the sensitivity of Deep Blue by an additional water wash step to detect even the weakest bands. Deep Blue offers the best results for the best price!
The reusable Deep Blue is stable at room temperature for at least one year. |
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| Two identical SDS polyacrylamide gels stained with Deep Blue (A) and
conventional Coomassie Blue + destain (B). |
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Product list with data sheets
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Deep Silver Stain - High-sensitive gel staining with one single solution!
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Features:
- Highly improved contrast and sensitivity (< 200 pg)
- Very fast and easy 30-minutes procedure!
- Suitable for 2D and DNA gels
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Example of a Deep Silver
stained SDS-PAGE gel. |
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Product description:
Deep Silver combines the sensitivity of conventional silver staining with the speed and ease you 've always been waiting for (see your bands 30 minutes after fixing). Whereas former protocols require multiple incubation, washing and development steps, Deep Silver does it all in one single solution! Moreover, contrast and sensitivity are markedly improved with this kit (down to 200 pg! ). While you got brownish bands on your gels until now, Deep Silver generates a deep black staining color. The silver stain develops over a period of 10 - 20 minutes, to give you enough time to stop the reaction at the point of staining intensity you require. The reaction is stopped in EDTA or standard fixing solution and gels can be dried and stored as usual. Deep Silver is suitable for the staining of 2D and DNA gels as well. One kit is sufficient for 40 SDS mini gels. |
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Product list with data sheets
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Snow White Reversible Zinc Stain - Rapid negative stain for SDS gels
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Features:
- Crystal clear protein bands
- Fast and simple two-step staining procedure
- Stained gel can be used for many subsequent procedures such as Western blotting, amino acid analysis, electroelution and MALDI-TOF
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| Example of a Snow White stained SDS-PAGE gel placed against the dark background of a UV-transilluminator. |
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Product description:
Snow White is a reversible, rapid negative stain for SDS-PAGE gels. A simple two-step staining procedure allows visualization of bands in just 10 minutes with a sensitivity close to that of silver stain. Snow White produces an opaque white background with crystal clear protein bands that can easily be viewed and documented when placed against a dark background (e.g. a UV screen). The destaining solution of the kit allows complete destaining of the gel in just 15 minutes. Gels can then be applied to subsequent procedures such as Western blotting, electroelution, amino acid analysis,
N-terminal sequencing, MALDI-TOF...you name it! |
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Product list with data sheets
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Enlight Fluorographic Enhancer - fluorographic enhancement of low-energy isotopes in polyacrylamide and agarose gels
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Product description:
Enlight is a non-toxic aqueous solution that allows the fluorographic enhancement of low-energy isotopes in polyacrylamide and agarose gels. After electrophoresis, the gel is soaked in the Enlight solution for one hour. Then, the gel is dried as usual and exposed to an X-ray film at -70°C. Low-energy electrons that cannot leave the gel matrix are likely to interact with fluorographic molecules which are now incorporated in the gel and stimulate a light-emitting process. These photons can easily leave the gel to expose the film. The increased detection efficiency for 3H is at least 10,000 fold, for 14C, 35S and 33P at least 100-fold! Enlight is stable at room temperature for at least 12 months. |
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Comparison of Enlight-treated and -un-treated gels with 14C-labeled protein marker and 3H- and 35S-labeled cell lysates after overnight exposure at -70°C. |
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Product list with data sheets
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NOWA Chemiluminescent Western - Highly sensitive detection system for
HRP-conjugated antibodies
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Product description:
NOWA (New Optical Western Analysis) is a highly sensitive detection system for HRP-conjugated antibodies in the picogram range. It enables a sensitivity that is significantly higher than that of other leading chemiluminescence kits. |
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| Comparison of light emission of NOWA and popular supplier A´s kit. |
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The kit is based on the emission of light during a reaction which is started by free oxygen produced by the enzymatic activity of HRP. Using a newly developed enhancer cascade, the light emission is amplified by a factor of at least one thousand! The NOWA kit contains two solutions that are combined and used for incubating the nitrocellulose or PVDF filter. After one minute of incubation time the filter is ready to be exposed to an X-ray film. Typical exposure times range between 10 seconds and 5 minutes. The light-emitting reaction continues for about 1 hour to allow as many exposures as you may need. The shelf life of the NOWA kit is at least 12 months when stored at 4°C. The kit contains 2 x 250 ml and is sufficient for 10,000 cm2 (1 m2 ) of NC or PVDF membrane.
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Product list with data sheets
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Order Information
| Click on the respective order # to download a detailed data sheet. |
| ORDER INFORMATION, SHIPPING & STORAGE: |
| order# |
description |
amount |
| DB001 |
Deep Blue (50 mini gels) |
0.5 l |
| DB002 |
Deep Blue (100 mini gels) |
1 l |
| SS001 |
Deep Silver Kit (40 mini gels) |
1 |
| shipped at RT; store at 4°C (Deep Blue can be stored at RT) |
| SW001 |
Snow White Kit (25 mini gels) |
1 |
| ENL01 |
Enlight |
0.5 l |
| ENL02 |
Enlight |
1 l |
| NOWA01 |
NOWA Kit (2 x 250 ml) |
1 |
| shipped at RT; store at RT (NOWA can be stored at 4°C) |
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