Frequently used standard markers at high quality

products | nucleic acid and protein tools | JBSolution Detergent Test Kit

Nucleic Acid and Protein Tools

JBSolution Detergent Test Kit

Product Description

The JBSolution Detergent Test Kit has been designed to optimize solubilization of membrane proteins. Compounds assembled in the kit range from ionic and non-ionic to zwitter-ionic detergents. These detergents have non-denaturing as well as denaturing properties. The arrangement is based on years of experience.

The kit contains 4 ml stock solutions (4%; except sulfobetaine SB16 with a concentration of 2.5%) of 27 detergents and 3 buffers at 1 M concentration (Tris-HCl, NaHEPES, NaPB), each at two different pH-values (7.5 and 8.0). All solutions are sterile-filtered.

The Critical Micelle Concentration (CMC) is very important for the use of detergents. Below the CMC, the detergents bind to the biological membrane by partitioning into the lipid bilayer. By increasing the detergent concentration, the lipid bilayer will be saturated with detergents. Therefore, it is necessary to work at a detergent concentration above the CMC. For dissolving hydrophobic proteins out of the biological lipid bilayer, add the required amount of the detergent stock solution to the buffer until the CMC is surpassed (the respective CMC values are given in the enclosed data sheet).

JBSolution Detergent Test Kit

Detergents provided with the kit:

cationic:

Cetylpyridinium chloride
Cetyltrimethylammonium bromide

non-ionic:

Brij 35®
Deoxy-BIGCHAP
HECAMEG
MEGA-8
MEGA-9
MEGA-10
Pluronic F-68
n-Octyl-beta-D-glucopyranoside
Sucrose monolaurate
Nonidet P40
Triton X-100
Triton X-114
Tween 20
Tween 80

anionic:

N-Lauroylsarcosin-sodium salt
Lithiumdodecyl sulfate
Sodium cholate
Sodium deoxycholate
SDS (Sodiumdodecylsulfate)

zwitter-ionic:

CHAPS
CHAPSO
Sulfobetaine SB10
Sulfobetaine SB12
Sulfobetaine SB14
Sulfobetaine SB16

For a detailed data sheet as a pdf-file, please click on the product No.

ORDER INFORMATION, SHIPPING & STORAGE:
order#	description	amount
DK-101JB	JB Solution Detergent Test Kit	1 kit
shipped on blue ice; store at 4°C