Re-usable compact reaction columns (CRC) -a unique technology!

products | nucleic acid and protein tools | Immobilized Enzymes for Solid Phase Enzyme Reactions

Nucleic Acid and Protein Tools

Immobilized Enzymes for Solid Phase Enzyme Reactions

Using our immobilized enzymes in compact reaction columns (CRC) saves your time in many molecular biological applications.

Features:

high enzyme concentration and activity
skip extensive steps in lab protocols
convenience and ease of handling
omit phenol extractions
avoid contaminating the lab (and your reaction solutions) with enzymes (RNase!)
enjoy short reaction and treatment times
get absolutely clean solutions
re-usability - use CR columns over and over again
take CR columns even for tiny solution volumes (20 µl!)
retain small volumes even after treatment
treat larger volumes using syringes or tubing with standard Luer-lock connections

General Product Description:

Compact reaction columns are small volume columns (Mobicols) containing a matrix to which enzymes are bound (immobilized covalently). Enzyme reactions occur when the substrate is added to the column. The sample is recovered from the column quantitatively by washing or centrifugation. The enzyme remains bound to the column.

Our Immobilization Technology was developed in co-operation with the Max Planck Society and is patented. It enables us to offer enzymes immobilized on both polyvinyl and dextran matrix. The immobilized enzymes retain their full activity and possess an extremely high occupational density.

Immobilized enzymes are supplied in extremely versatile compact reaction columns (CRC) which fit into 1.5 ml microcentrifuge tubes. The columns have Luer-lock fittings, allowing direct syringe application of substrate solution, continuous flow processing of bulk solutions, or application of pressure for recovering the substrate. For small substrate volumes (approx. 50 µl or less), most enzyme columns can be spun dry in benchtop centrifuges for fast, effective recovery.

The immobilized enzymes are extremely stable in aqueous media over a pH range of 5 to 10 and column bleeding is negligible. The "stiff" linkers which separate the enzyme from the matrix surface effectively eliminate steric hindrance. As a result, the enzymes retain essentially their full activity in the immobilized state.

This well established technology allows you to expose your reaction solutions to very high concentrations of modifying enzymes. The exposure to high enzyme concentrations allows reaction times to be greatly reduced.

Two different immobilization matrices are available: F7m and G3m. They have different pore characteristics:

Matrix F7m has large pores. Molecules with up to 10⁷ Dalton molecular weight (most enzymes and substrates) can enter these pores. The total surface of the material is very large, resulting in an extremely high enzyme activity on the matrix.
G3m has extremely small pores; this results in excellent recovery characteristics (i.e. complete recovery in very small volumes). Molecules larger than 10³ Dalton molecular weight (larger peptides, proteins and nucleic acids) cannot enter these pores. The total surface area of the material is smaller than for F7m resulting in a smaller enzyme activity on the matrix.

ORDER INFORMATION, SHIPPING & STORAGE:
order#	description	amount
Immobilized Proteinases and Inhibitors (in CRC)
P5101	Endoproteinase Glu-C (Prot.V8) (one column with 200 µl matrix F7m)	900 U
P3102	Endoproteinase Glu-C (Prot.V8) (one column with 200µl matrix G3m)	22 U
P5401	Papain (one column with 200 µl matrix F7m)	23 U
P3402	Papain (one column with 200 µl matrix G3m)	0.6 U
P5121	Pepsin (one column with 200 µl matrix F7m)	16 mAnson U
P3122	Pepsin (one column with 200 µl matrix G3m)	0.4 mAnson U
P5501	Proteinase K (one column with 200 µl matrix F7m)	27 mAnson U
P3502	Proteinase K (one column with 200 µl matrix G3m)	0.7 mAnson U
P5301	TLCK-α-Chymotrypsin (one column with 200 µl matrix F7m)	55 U
P3302	TLCK-α-Chymotrypsin (one column with 200 µl matrix G3m)	1.4 U
P5701	TPCK-Trypsin (one column with 200 µl matrix F7m)	10,200 St-U
P3702	TPCK-Trypsin (one column with 200 µl matrix G3m)	260 St-U
Immobilized Nucleases (in CRC; except N 3403)
N5401	DNase I (one column with 200 µl matrix F7m)	3,500 U
N3402	DNase I (one column with 200 µl matrix G3m)	88 U
N3403	DNAse I (kit with 200 µl G3m matrix and 5 empty columns)	88 U
N5101	RNase A (one column with 200 µl matrix F7m)	50 Kunitz-U
N3102	RNase A (one column with 200 µl matrix G3m)	2.5 Kunitz-U
N5201	RNase T1 (one column with 200 µl matrix F7m)	176,000 U
N3202	RNase T1 (one column with 200 µl matrix G3m)	8,800 U
Immobilized Other Enzymes (in CRC)
A5201	Alkaline phosphatase (CIP) (one column with 200 µl matrix F7m)	1,000 U
A3202	Alkaline phosphatase (CIP) (one column with 200 µl matrix G3m)	100 U
A5101	beta-Galactosidase (one column with 200 µl matrix F7m)	600 U
A3102	beta-Galactosidase (one column with 200 µl matrix G3m)	15 U
shipped at RT; store at 4°C; * not available in the US

Downloads: Handbook

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