products | DNA vector systems | Standard Cloning Vectors

DNA Vector Systems

Standard Cloning Vectors

Features:

• highest quality DNA prepared by
  • ion-exchange chromatography,
  • cesium chloride density centrifugation and
  • gel filtration
• preparations with over 80% supercoiling
• of special interest: pUC118 and pUC119
• ready-to-use for:
  • transformations
  • enzymatic reactions
• competitive price!
  

pBR322:

pBR322 is an ampicillin and tetracycline resistant, general purpose cloning vector. Several inactivating cloning sites are present in the antibiotics resistance genes, which, if a reading frame shift occurs, will give rise to Amp^R /Tc^S or, alternatively, Amp^S /Tc^R transformants. Cloning sites are indicated on the data sheet.

pBR325:

pBR325 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. It is derived from pBR322 by insertion (into its EcoR I site) of the chloramphenicol acetyltransferase gene.

pBR328:

pBR328 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. This vector, derived from pBR325, has the bom site (basis of mobility) deleted and therefore is non-mobilizable; this makes pBR328 suitable where more stringent biological containment is required. This deletion also creates extra unique cloning sites in the chloramphenicol acetyltransferase gene: Pvu II, BspM II and Bal I.

pUC18/19 and pUC118/119:

The plasmids have a multiple cloning site within the lacZ alpha-fragment. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-Gal/IPTG plates. The plasmids encode resistance to ampicillin. Foreign DNA inserted in-frame with the lac Z gene will be expressed as a fusion protein (containing a portion of the beta-galactosidase) under control of the lac promoter. The promoter is inducible with IPTG and followed by an initiation codon as well as a ribosome binding site. pUC18 and pUC19 differ in their multiple cloning site orientation. pUC118 and pUC119 contain an additional M13 phage origin for single strand production.

pACYC184:

pACYC184 encodes tetracycline and chloramphenicol resistance. Unlike most cloning vectors, which have ColE1 origins of replication, pACYC184 has an origin derived from p15A1. This allows pACYC184 to be maintained in a pBR322 or pUC18 transformant, for example. Such a double transformant is necessary where two recombinant proteins need to be expressed simultaneously.

pAT153:

pAT153 is a derivative of pBR322 where the bom (basis of mobility) site has been deleted. Thus pAT153 is non-mobilizable and is more readily contained than pBR322. Also in this 703 bp deletion was the region involved in copy number control; loss of this region gives pAT153 a 1.5 to 3-fold higher copy number. pAT153 encodes ampicillin and tetracycline resistance.