The ultimate multiple cloning site: contains 59 unique restriction sites

products | DNA vector systems | Multiple Cloning Site Vector pMCS5

DNA Vector Systems

Multiple Cloning Site Vector pMCS5

Features:

standard cloning into a site upstream from a T7-RNA-polymerase-promoter
end-cloning
labeling
linearization of recombinant clones
blue/white selection
generation of single-strand DNA (using the f1 origin)

Product Description:

The new cloning vector pMCS5 contains the ultimate multiple cloning site (MCS) with 59 unique restriction sites. Included are the recognition sites for many commonly used restriction enzymes, providing a suitable cloning site for almost any application.

The scientist can chose between 46 unique hexamer sites, two heptamer sites and the recognition sites for all ten known octanucleotide-specific endonucleases. In addition, the well-defined 18-mer sequence of the extremely rarely cutting enzyme I-Sce I is located at one terminus of the MCS, enabling the linearization of recombinant clones. I-Sce I does not recognize a palindrome and can thus be used for labeling and end-cloning. Further it can be utilized for strand protection in unidirectional deletion experiments. Included in the MCS are also restriction sites which occur very infrequently in human DNA such as Mlu I, Nru I and Spl I.

Restriction Enzyme Recognition sites in the 259-bp MCS cassette

Recognition Sequences:	Enzymes:	Properties:
Heptamer sequences	Bae I,RSr II
Octamer sequences	Asc I, Fse I, Not I, Srf I Pac I, Swa I Pme I, SgrA I, Sse8387 I, Sfi I	only GC pairs only AT pairs
Hexamer sequences	Mlu I, Nru I, Spl I, Cla I Acc I, Acc I, Age I, Apa I, Avr II, BamH I, Bbe I, Bgl II, Bsp120 I, Bsp I, BspM I, BssH II, BstB I, Ecl136 II, EcoR I, EcoR V, Ehe I, Hind III, Hpa I, Kas I, Kpn I, Mun I, Nar I, Nco I, Nde I, Nhe I, Nsi I, Nsp7524 V, Pml I, Ppu I, Pst I, Sac I, Sac II, Sal I, Sma I, SnaB I, Spe I, Sph I, Xba I, Xho I, Xma I, Xma III	very infrequent in human DNA since the plasmid was produced in the dam⁺ strain DH5alpha ,the Cla I site is methylated and cannot be cleaved
18-mer sequence	I-Sce I	highly unlikely in any DNA

ORDER INFORMATION, SHIPPING STORAGE:
order#	description	amount
PMCS5	pMCS5 vector DNA, lyophilized	5 µg
shipped at RT; store at 4°C

Downloads: pMCS5 sequence, Handbook

For further Vector System products please download the Vector Systems brochure (PDF, 3,9 MB)

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