products | TefLabs
 |
TefLabs
TefLabs is a research driven company formed in 1992 by Dr. A. Minta, Ph.D to provide scientists worldwide with a choice for better fluorescent indicators at reasonable and affordable costs. This drive has lead TEF Labs to develop new products that satisfy the deficiencies of current technologies by designing fluorescent and molecular probes that have better quantum yield, improved sensitivity and selectivity, reduced photobleaching, longer wavelengths, leakage resistance and ratiometric properties.
TefLabs products are currently being utilized in the area of Biochemistry, Biomolecular screening, Cell Biology, High throughput Screening, Microbiology, Molecular Biology and Neuroscience.
 |
Ion Indicators |
|
General Fluorophores |
Ion Indicators
Calcium Indicators
We offer Bapta, Quin-2, Fura-2, Indo-1 and Rhod-2.
Fura-2 continues to be the most popular dye because of the shift in excitation wavelength which allows for ratio measurements. The ratiometric property eliminates variable effects such as cell loading, photobleaching, detector sensitivity, cell thickness and optical path. Indo-1 is the next most popular dye, and is also ratiometric. The syntheses of these two dyes have been known for over twelve years and have been done in our labs so often that we can purify them to 100% by HPLC. Both dyes have their absorption in the UV region.
Bapta and Quin-2 were the first usable calcium indicators and they are still popular for specific applications. All these dyes are available in HPLC grade purity and are reasonably priced.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0100 | Bapta(AM) | 765 | 25 mg |
| 0101 | Bapta(K+ -Salt) | 629 | 1 g |
| 0102 | Fura-2(AM) | 1002 | 1 mg |
| 0103 | Fura-2(AM) | 1002 | 20 x 50 µg |
| 0104 | Fura-2(K+ -Salt) | 832 | 1 mg |
| 0105 | Indo-1(AM) | 1010 | 1 mg |
| 0106 | Indo-1(AM) | 1010 | 20 x 50 µg |
| 0107 | Indo-1(K+ -Salt) | 840 | 1 mg |
| 0114 | Quin-2(AM) | 830 | 10 mg |
| 0115 | Quin-2(K+ -Salt) | 542 | 5 mg |
| 0119 | Rhod-2(AM) | 1124 | 1 mg |
| 0120 | Rhod-2(AM) | 1124 | 20 x 50 µg |
| 0121 | Rhod-2(K+ -Salt) | 869 | 1 mg |
Leakage Resistant Calcium Indicators
Please download more information on Leakage Resistant Calcium Indicators.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0108 | Fura-PE3(AM) | 1258 | 500 µg |
| 0109 | Fura-PE3(AM) | 1258 | 10 x 50 µg |
| 0110 | Fura-PE3(K+ -Salt) | 1054 | 500 µg |
| 0144 | Indo-PE3(AM) | 1266 | 500 µg |
| 0145 | Indo-PE3(AM) | 1266 | 10 x 50 µg |
| 0146 | Indo-PE3(K+-Salt) | 1062 | 500 µg |
Near Membrane Calcium Indicators
The publication by Fred Fay describes it as the best near membrane indicator ever made:
Etter, E., Minta, A., Poenie, M., & Fay, F. (1996) Near Membrane Ca2+ Transients Resolved Using the Ca2+ Indicator FFP18. Proceedings of the National Academy of Sciences 93, 5368-5373.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0111 | FFP-18(K+ -Salt) | 1164 | 250 µg |
| 0125 | FFP-18(AM) | 1296 | 500 µg |
| 0126 | FFP-18(AM) | 1296 | 10 x 50 µg |
| 0127 | FIP-18(AM) | 1303 | 500 µg |
| 0128 | FIP-18(AM) | 1303 | 10 x 50 µg |
| 0129 | FIP-18(K+ -Salt) | 1134 | 250 µg |
High Calcium Indicators
Please download detailed information on High Calcium Indicators.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0122 | 5,5' difluoro-Bapta(AM) | 801 | 10 mg |
| 0123 | 5,5' difluoro-Bapta(K+-Salt) | 655 | 50 mg |
| 0135 | Fura-2FF(AM) | 1038 | 500 µg |
| 0136 | Fura-2FF(AM) | 1038 | 10 x 50 µg |
| 0137 | Fura-2FF(K+-Salt) | 868 | 500 µg |
| 0138 | Indo-1FF(AM) | 1046 | 500 µg |
| 0139 | Indo-1FF(AM) | 1046 | 10 x 50 µg |
| 0140 | Indo-1FF(K+Salt) | 876 | 500 µg |
| 0141 | Fluo-3FF(AM) | 1166 | 500 µg |
| 0142 | Fluo-3FF(AM) | 1166 | 10x50 µg |
| 0143 | Fluo-3FF(K+-Salt) | 996 | 500 µg |
| 0147 | Bapta-FF(Free Acid) | 477 | 10 mg |
| 0148 | Bapta-FF (AM) | 766 | 10 mg |
Zinc
We offer TSQ9, the most common stain for Zinc, developed by Dr. Fredrickson. In addition, we have developed a water soluble version TFLZn which has found a great application in the imaging of zinc in synaptic terminals of live hippocampal slices by Dr. Alan Kay.
Budde, T., Minta, A., White, J.A., & Kay, A.R. (1997) Imaging Free Zinc in Synaptic Terminals in Live Hippocampal Slices. Neuroscience 79, 347-358.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0010 | TSQ | 328 | 25 mg |
| 0011 | TFLZn(K+Salt) | 416 | 5 mg |
| 0012 | TFLZn(Free Acid) | 378 | 5 mg |
| 0013 | TFLZn(AM) | 450 | 5 mg |
| 0014 | Zinquin Free Acid | 386.42 | 5 mg |
| 0015 | Zinquin (AM) | 458.49 | 5 mg |
Potassium
We offer PBFI for potassium ion. We offer custom synthesis of any of these compounds as contained in the classic paper:
Minta, A., & Tsien, R.Y. (1989) Fluorescent Indicators for Cytosolic Sodium. Journal of Biological Chemistry 264(32), 19449-19457.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0020 | PBFI(AM) | 1171 | 1 mg |
| 0021 | PBFI(AM) | 1171 | 20 x 50 µg |
| 0022 | PBFI(TMA Salt) | 1175 | 1 mg |
Sodium
We offer SBFI for sodium ion. We offer custom synthesis of any of these compounds as contained in the classic paper:
Minta, A., & Tsien, R.Y. (1989) Fluorescent Indicators for Cytosolic Sodium. Journal of Biological Chemistry 264(32), 19449-19457.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0030 | SBFI(AM) | 1127 | 1 mg |
| 0031 | SBFI(AM) | 1127 | 20 x 50 µg |
| 0032 | SBFI(K+-Salt) | 1131 | 1 mg |
Magnesium
Furaptra2 or Magfura and Mag-Indo are fura and indo versions of a partial Bapta that work well as magnesium indicators. These are available in the form of potassium salt and the acetoxymethyl ester. We now have the fluo-3 version for measurement with visible light. For a long time these dyes were used for measuring high calcium but our recent offering of the FF series is allowing scientists to restrict their use to magnesium.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0040 | Furaptra(AM) | 723 | 1 mg |
| 0041 | Furaptra(AM) | 723 | 20 x 50 µg |
| 0042 | Furaptra(K+-Salt) | 587 | 1 mg |
| 0043 | Indo-Mag(AM) | 615 | 1 mg |
| 0045 | Indo-Mag(AM) | 615 | 20 x 50 µg |
| 0046 | Indo-Mag(K+-Salt) | 513 | 1 mg |
Chloride
The chloride indicator SPQ7, developed by Dr. Wolfberg as a standard for fluorescent measurements, has been used successfully by Dr. Verkman as a chloride-quenching indicator:
Illsley, N.P., & Verkman, A.S. (1987) Membrane Chloride Transport Measured Using a Chloride Sensitive Fluorescent Probe. Biochemistry 26, 1215-1219.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0050 | SPQ | 281 | 100 mg |
| 0051 | MQAE | 100 mg |
pH Indicators
BCECF1 still remains the indicator of choice for measuring pH in cells, primarily because of its ideal pKa and its ratioability. BCECF is available from Tef Labs in the free acid form and the acetoxymethyl ester form.
Rink, T.J., Tsien, R.Y., & Pozzan, T. (1982) Cytoplasmic pH and Free Magnesium in Lymphocytes. Journal of Cell Biology 95, 189-196.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0060 | BCECF(Free Acid) | 520 | 1 mg |
| 0061 | BCECF(AM) | 821 | 1 mg |
| 0062 | BCECF(AM) | 821 | 20 x 50 µg |
Ionophores
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0070 | SQI-Et (Na) | 676 | 500 µg |
| 0071 | SQI-Pr(Na) | 732 | 500 µg |
| 0071SP | SQI-Pr (Na) special packaging | 732 | 5 mg |
| 0072 | A23187(Ca) | 524 | 10 mg |
| 0073 | 4-BrA23187(Ca) | 602 | 1 mg |
pKC Indicators
Please download more information on PKC Indicators.
For a detailed data sheet as a pdf-file, please click on the product No.
| order# | product | MW | unit size |
| 0080 | FIM-1 (k+-Salt) | 851 | 250 µg |
| 0081 | RIM-1 | 953 | 250 µg |
| 0082 | RIM-1 (Diacetate) | 843 | 250 µg |
Also available: Pluronic
The nonionic surfactant polyol (MW approx. 12,500 Da) facilitates the solubilization of water-insoluble dyes in physiological media. Pluronic helps to disperse the acetoxymethyl (AM) esters ion indicators as well as cell tracer dyes such as CFDA-SE.
| order# | Pproduct | unit size |
| 2510 | Pluronic® 20% solution in DMSO | 1 ml |
For more information on Teflabs products please visit the Teflabs web-page
|
home | search | site map | order | Impressum | ©2008 MoBiTec GmbH • All rights reserved • www.mobitec.com |