products | Molecular Probes

Probes for Cell Organelles

For more information please browse the ninth edition of the famous fluorescent handbook online.
 

Please view some product highlights:

	TubulinTrackerTM Green Reagent
	ER-TRackerTM Green & Red Dyes
	SelectFXTM Alexa Fluor® 488 ER Labeling Kit
	SelectFXTM Nuclear Labeling Kit

For a detailed data sheet as a pdf-file, please click on the product No.

ER-TRacker^TM Green & Red Dyes

For live cell endoplasmatic reticulum staining

The green-fluorescent ER-Tracker Green and red-fluorescent ER-Tracker Red dyes (E34251, E34250) correspond to the drug conjugates BODIPYFL glibenclamide and BODIPY TR glibenclamide, which exhibit excitation/emission maxima of ~540/511 nm and 587/615 nm, respectively. Glibenclamide (glyburide), a drug taken daily by millions of diabetic patients to correct hyperglycemia, has been used to investigate pancreatic-cell activity and insulin secretion and to study myocardial cell function and heart arrhythmia. Glibenclamide binds to the sulphonylurea receptors of ATP-sensitive K+ channels, which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

• correspond to the drug conjugates BODIPY^® FL and BODIPY^® TR glibenclamide
• Ex/Em maxima of 504%511 and 587/615 nm, respectively

SelectFX^TM Alexa Fluor^® 488 ER Labeling Kit

The SelectFX Alexa Fluor^® 488 Endoplasmic Reticulum Labeling Kit (S34200) provides all the reagents required to fix and permeabilize mammalian cells and then specifically label the ER. To achieve ER labeling, this kit employs a primary antibody directed against an ER-associated protein, protein disulfide isomerase (PDI), and an Alexa Fluor^® 488 dye-labeled secondary antibody. The Alexa Fluor^® 488 dye exhibits bright green fluorescence that is compatible with filters and instrument settings appropriate for fluorescein.

Each kit contains:

• Mouse IgG2b anti-protein disulfide isomerase (PDI) antibody
  
• Highly cross-adsorbed Alexa Fluor 488 goat anti-mouse IgG antibody
  
• Concentrated fixative solution
  
• Concentrated phosphate-buffered saline (PBS)
  
• Concentrated permeabilization solution
  
• Concentrated blocking solution
  
• Detailed protocols for mammalian cell preparation and staining
  

The SelectFX Alexa Fluor^® 488 Endoplasmic Reticulum Labeling Kit can be used in conjunction with probes for other cell targets to achieve multicolor cell staining.

SelectFX^TM Nuclear Labeling Kit

Fixed, permeabilized bovine pulmonary artery endothelial cells visualized using components of the SelectFX™ Nuclear Labeling Kit and Alexa Fluor® phalloidin conjugates. Nuclei and F-actin were stained, respectively, with (top left) DAPI & Alexa Fluor® 680 phalloidin, (top right) SYTOX® Green dye & Alexa Fluor® 568 phalloidin, (bottom left) 7-AAD & Alexa Fluor® 488 phalloidin, and (bottom right) TO-PRO-3 dye & Alexa Fluor® 350 phalloidin.

Highly sensitive nuclei staining

The SelectFX^TM Nuclear Labeling Kit (S33025) provides four spectrally distinct fluorescent dyes - blue-fluorescent DAPI, green-fluorescent SYTOX Green stain, red-fluorescent 7-aminoactinomycin D (7-AAD) and far red-fluorescent TO-PRO-3 dye - for staining nuclei in fixed and permeabilized cells and tissues with essentially no cytoplasmic background staining. When used according to the protocol provided, the dyes in the SelectFX^TM Nuclear Labeling Kit provide highly selective nuclear staining with little or no cytoplasmic labeling; they are ideal for use as counterstains in multicolor applications. The stained nuclei stand out in vivid contrast to other fluorescently labeled cell structures when observed by fluorescence microscopy. These dyes have excitation wavelengths that match the common laser lines for confocal microscopy and flow cytometry and can be used with standard filter sets on fluorescence microscopes and microplate readers. The staining protocol provided is compatible with a wide range of cytological labeling techniques, including direct or indirect antibody-based detection methods, mRNA in situ hybridization and staining methods that incorporate organelle- and cytoskeleton-selective fluorescent probes (including MitoTracker mitochondrion-selective probes and Alexa Fluor^® dye conjugated phalloidins). The dyes can also be used to fluorescently stain cells for analysis in multicolor flow cytometry experiments. All dyes are provided as stock solutions, convenient for diluting and staining, and each dye is also available separately.

The SelectFX Nuclear Labeling Kit contains:

• DAPI, a blue-fluorescent counterstain (excitation/emission maxima ~358/451 nm)
  
• SYTOX Green, a green-fluorescent counterstain (excitation/emission maxima ~504/523 nm)
  
• 7-Aminoactinomycin D (7-AAD), a red-fluorescent counterstain (excitation/emission maxima ~546/647 nm)
  
• TO-PRO-3 iodide, a far red-fluorescent counterstain (excitation/emission maxima ~642/661 nm)
  
• Detailed staining protocols (SelectFX^TM Nuclear Labeling Kit)